CKM Protein
Quick Overview for CKM Protein (ABIN934527)
Target
See all CKM ProteinsProtein Type
Origin
Source
Purity
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Characteristics
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Purified recombinant CKMM protein
Expression System: Yeast
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Creatine Kinase, Muscle (CKM) (AA 1-381) protein (His tag)
custom-made CKM Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Creatine Kinase, Muscle (CKM) (AA 1-162) protein (His tag)CKM Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Creatine Kinase, Muscle (CKM) (AA 1-381) protein (His tag)CKM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Creatine Kinase, Muscle (CKM) (AA 1-381) protein (GST tag)CKM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Buffer
- Tris buffered saline, pH 7.2, with 10 mM beta-Mercaptoethanol, 50 % glycerol and 0.1 % NaN3.
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Preservative
- Sodium azide
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -80 °C
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Storage Comment
- Aliquot and store at -70 °C or lower.
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- CKM (Creatine Kinase, Muscle (CKM))
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Alternative Name
- CKMM
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Background
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Creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are, therefore, three different isoenzymes: CK-MM, C kbB and CK-MB. .
Alternative Names: CK-MM protein, Creatine Kinase-MM Isoenzyme Protein -
Molecular Weight
- 43 kDa
Target
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