HIV-1 Transmembrane Glycoprotein (HIV-1 gp41) Protein

Catalog No. ABIN934586

Product Details

Target: HIV-1 Transmembrane Glycoprotein (HIV-1 gp41)

Protein Type: Recombinant

Origin: Human Immunodeficiency Virus (HIV)

Source: Escherichia coli (E. coli)

Characteristics: Purified recombinant HIV1 gp41 protein
Expression System: E.coli

Purity: > 90 % pure

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Buffer: 50 mM borax buffer, pH 9.0, with 0.02 % NaN3.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References

Du, Idiart, Mariano, Chen, Jiang, Xu, Ostrow, Wrin, Phung, Binley, Petropoulos, Ballantyne, Whalen: "Effect of trimerization motifs on quaternary structure, antigenicity, and immunogenicity of a noncleavable HIV-1 gp140 envelope glycoprotein." in: Virology, Vol. 395, Issue 1, pp. 33-44, (2009) (PubMed).

Opalka, Pessi, Bianchi, Ciliberto, Schleif, McElhaugh, Danzeisen, Geleziunas, Miller, Eckert, Bramhill, Joyce, Cook, Magilton, Shiver, Emini, Esser: "Analysis of the HIV-1 gp41 specific immune response using a multiplexed antibody detection assay." in: Journal of immunological methods, Vol. 287, Issue 1-2, pp. 49-65, (2004) (PubMed).

Target Details

Target: HIV-1 Transmembrane Glycoprotein (HIV-1 gp41)

Alternative Name: HIV1 gp41

Target Type: Viral Protein

Background: HIV gp41 is a glycoprotein subunit that remains non-covalently-bound to gp120, and provides the second step by which HIV enters the cell. It is originally buried within the viral envelope, but, when gp120 binds to a CD4, gp120 changes its conformation, causing gp41 to become exposed, where it can assist in fusion with the host cell. The Env gene does not, in fact, code for gp120 and gp41, but for a precursor to both, gp160. During HIV reproduction, the host cell's own enzymes cleave gp160 into gp120 and gp41.
Alternative Names: gp41 antigen, Envelope surface glycoprotein gp41 antigen, HIV-1 gp41, gp41 antigen, HIV 1 gp41, gp41 glycoprotein antigen, SU antigen, HIV 1 gp41 protein, Surface protein antigen, HIV1 gp41, HIV-1 gp41 antigen, Glycoprotein 41 antigen, Human Immunodeficiency Virus 1 antigen, HIV-1 gp41 protein, HIV 1 gp41 antigen