proBNP Protein
Quick Overview for proBNP Protein (ABIN934800)
Target
Protein Type
Origin
Source
Purity
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Characteristics
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Purified recombinant Human ProBNP protein
Expression System: E.coli -
Purification
- Immunoaffinity purification followed by ion exchange chromatography
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Pro-Brain Natriuretic Peptide (proBNP) (AA 27-121) protein (His tag)
proBNP Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Pro-Brain Natriuretic Peptide (proBNP) (AA 1-76) protein (His tag)proBNP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Buffer
- 10 mM KH2PO4, pH 7.4, 150 mM NaCl. No preservatives added. To prepare diluted samples of proBNP it is recommended to use buffer solutions containing carrier protein and detergent.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -80 °C
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Storage Comment
- Aliquot and store at -70 °C or lower.
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- proBNP (Pro-Brain Natriuretic Peptide (proBNP))
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Alternative Name
- ProBNP
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Background
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Brain natriuretic peptide (BNP), now known as B-type natriuretic peptide (also BNP) or GC-B, is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). The physiologic actions of BNP are similar to ANP and include decrease in systemic vascular resistance and central venous pressure as well as an increase in natriuresis. proBNP is the prohormone form of BNP.
Alternative Names: pro Brain Natriuretic Peptide Protein, NT-proBNP Protein, ProBNP, NPPB Protein
Target
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