SPARC Protein
Quick Overview for SPARC Protein (ABIN934839)
Target
See all SPARC ProteinsProtein Type
Origin
Source
Purity
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Characteristics
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Purified native Human Osteonectin protein
Protein Source: Human platelets
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) protein (His tag)
SPARC Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) protein (His tag)SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) protein (His tag)SPARC Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Buffer
- 20 mM Tris, 150 M NaCl, pH 7.4.
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Precaution of Use
- Donor samples were tested and found to be negative for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II. Nonetheless caution should be used when handling this material as there is a margin of error in all tests.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20 °C.
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Alternative Name
- Osteonectin
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Background
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Osteonectin is an acidic, noncollagenous glycoprotein originally isolated from fetal and adult bovine bone matrix. In vitro bovine bone osteonectin binds type I collagen, calcium (kDa=3x10-7 M) and hydroxyapatite (kDa=8x10-8 M) and has been shown to be a potent inhibitor of hydroxyapatite seeded crystal growth. In this context it has been suggested that osteonectin may play an important role in the regulation of bone metabolism by binding hydroxyapatite to collagen.
Description: Human platelets. -
Molecular Weight
- 32.7 kDa
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Pathways
- Autophagy
Target
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