UBE2I Protein

Catalog No. ABIN934907

This Recombinant UBE2I protein is produced in Escherichia coli (E. coli).

Quick Overview for UBE2I Protein (ABIN934907)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS

Characteristics: Purified recombinant Human Ubc9 protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: Ubiquitin protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 50 mM HEPES, pH7.5, containing 150 mM NaCl, 0 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: Ubc9

Background: Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes.
Alternative Names: EC 6.3.2 protein, UBE 2I protein, Ubiquitin conjugating enzyme UbcE2A protein, SUMO-conjugating enzyme UBC9 protein, Ubiquitin like protein SUMO 1 conjugating enzyme protein, Ubiquitin protein ligase E2I protein, Ubiquitin carrier protein 9 protein, UBCE 9 protein, SUMO1 protein ligase protein, Ubiquitin like protein SUMO1 conjugating enzyme protein, SUMO protein ligase protein, Ubiquitin protein ligase I protein, Human ubiquitin conjugating enzyme 9 protein, UBC9 homolog protein, p18 protein, SUMO 1 conjugating enzyme protein, , Ubiquitin conjugating enzyme E2I protein, UBCE9 protein, SUMO 1 protein ligase protein, Ubiquitin-conjugating enzyme E2I C358B7.1 protein, Ubiquitin-protein ligase I protein, UBC 9 protein, Ubiquitin carrier protein I protein, Ubiquitin carrier protein protein, SUMO1 conjugating enzyme protein, Ubiquitin conjugating enzyme 9 protein, p18 protein, Homologous to yeast UBC9 protein, UBE2I protein, Ubc9 protein, UBC9 protein, SUMO-protein ligase protein, SUMO conjugating enzyme UBC9 protein, Ubiquitin-conjugating enzyme E2 I protein

Molecular Weight: 18 kDa (158 AA)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway