Ubiquitin Protein (Ub)

Catalog No. ABIN934908

This Recombinant Ubiquitin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Ubiquitin Protein (Ub) (ABIN934908)

Target: Ubiquitin (Ub)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Sequence: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Characteristics: Purified recombinant Human Ubiquitin protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: Ubiquitin protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 50 mM HEPES, pH7.5, containing 150 mM NaCl, 0 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone 2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity. Ubiquitin (76 amino acids) was overexpressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: UBCEP1 protein, UBA80 protein, UBCEP2 protein, UBC protein, Ubiquitin protein, Polyubiquitin B protein, UBB protein, FLJ25987 protein, Ubiquitin B protein, UBA52 protein, RPS27A protein, , MGC8385 protein

Molecular Weight: 8.6 kDa (76 AA)

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway