GrpE Protein

Catalog No. ABIN934953

This Recombinant GrpE protein is expressed in Escherichia coli (E. coli).

Quick Overview for GrpE Protein (ABIN934953)

Target: GrpE

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % pure

Product Details

Sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA

Characteristics: Purified recombinant E.coli GrpE protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: GrpE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris, pH 8.0, containing 100 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: GrpE

Background: GrpE ,co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.
Alternative Names: GrpE protein, HSP-70 cofactor protein, Heat shock protein B25.3 protein, HSP24 protein, HSP 70 cofactor.

Molecular Weight: 21.8 kDa (197 AA)