SlyD (AA 1-196) (Active) Protein

Catalog No. ABIN934975

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for SlyD (AA 1-196) (Active) Protein (ABIN934975)

Target: SlyD

Protein Type: Recombinant

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-196

Sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

Characteristics: Purified recombinant E.coli SlyD protein
Expression System: E.coli
Bioactivity: Specific activity is > 66 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: SlyD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
   2. Add recombinant SlyD protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
   3. Mix by inversion and equilibrate to 1C and monitor the A410 nm until the value is constant using a spectrophotometer.
   4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
   5. Record the increase in A410 nm for 20 minutes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris, pH 7.5.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: SlyD

Background: SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. coli slyD was expressed in E. coli and purified by conventional chromatography.
Alternative Names: WHP., FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD protein, Histidine rich protein protein, FKBP-type peptidyl-prolyl cis-trans isomerase protein, Rotamase protein, PPIase protein, SlyD protein

Molecular Weight: 21 kDa (196 AA)