SOD1 Protein (AA 1-154)

Catalog No. ABIN934983

This Recombinant SOD1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SOD1 Protein (AA 1-154) (ABIN934983)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-154

Sequence: MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ

Characteristics: Purified recombinant Human SOD1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 90 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50 % in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25 °C in a 1.5 ml reaction volume.
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: SOD1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare a 1.5 mL reaction mix in a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.0 mM ethylendiaminetetraacetic acid, 0.00 mM cytochromC 0.00 mM xanthine, 0.005 units xanthine oxidase.
   2. Equilibrate to 25 °C and monitor at A550 nm until the value is constant using a spectrophotometer.
   3. Add 50 µL of recombinant SOD protein in various concentrations (0.5 µg, 5 µg) in assay buffer.
   4. Mix by inversion and record the increase at A550 nm for 5 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl, pH 7.5, containing 10 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background: Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS). Recombinant SOD1 was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: SOD-1 protein, SOD protein, , Mn superoxide dismutase protein, SOD2 protein, SOD-1, SOD 1, Indophenoloxidase A protein, ALS protein, soluble ALS 1 protein, Superoxide dismutase 1 soluble protein, Superoxide dismutase 1 soluble protein, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult protein, Superoxide dismutase Cu Zn protein, Homodimer protein, SOD1, SODC protein, Cu/Zn superoxide dismutase protein, SOD1 protein, Cu-Zn superoxide dismutase protein, SOD 1 protein, IPOA protein, Superoxide dismutase cystolic protein, Cu/Zn SOD protein, SOD soluble protein, Superoxide dismutase 1 protein, ALS1 protein, SOD 1 protein

Molecular Weight: 15.9 kDa (154 AA)

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis