Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491)

Catalog No. ABIN935018

This Recombinant Glucose-6-Phosphate Dehydrogenase protein is produced in Escherichia coli (E. coli).

Quick Overview for Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491) (ABIN935018)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Protein Type: Recombinant

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % pure

Product Details

Protein Characteristics: AA 1-491

Sequence: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Characteristics: Purified recombinant E.coli G6 PD protein
Expression System: E.coli
Bioactivity: Specific activity is 8-10 units/mL obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 m D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C.
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: G6PD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare a 3 mL reaction cocktail into a suitable container: The final concentrations are 53 mM gylcylglycine, 3 mM D-glucose 6-phosphate, 0.63 mM beta-NADP, 13 mM MgCl2.
   2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
   3. Add 4 µg of recombinant G6PD into reaction cocktail and mix immediately.
   4. Record the increase at A340 nm for 5 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 mM MES 6.0, 0.0 mM PMSF, 0 mM EDTA, 0.0 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD

Background: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphogluconolactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: Glucose 6 phosphate 1 dehydrogenase protein, G6PD1 protein, zwf protein, GPD 6, Glucose-6-phosphate 1-dehydrogenase G6PD protein, Glucose-6-phosphate 1-dehydrogenase protein, G6PD, POS10 protein, GPD-6, GPD-6 protein, MET19 protein, GPD 6 protein, G6pdx protein, Zwf1p.

Molecular Weight: 55.7 kDa (491 AA)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones