Troponin C (AA 1-160) Protein

Catalog No. ABIN935041

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for Troponin C (AA 1-160) Protein (ABIN935041)

Target: Troponin C

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-160

Sequence: MTDQQAEARS YLSEEMIAEF KAAFDMFDAD GGGDISVKEL GTVMRMLGQT PTKEELDAII EEVDEDGSGT IDFEEFLVMM VRQMKEDAKG KSEEELAECF RIFDRNADGY IDPEELAEIF RASGEHVTDE EIESLMKDGD KNNDGRIDFD EFLKMMEGVQ

Characteristics: Purified recombinant Human Troponin C protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: Troponin C protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM DTT, 100 mM NaCl, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: Troponin C

Background: Troponin C, skeletal muscle, also known as TNNC2, is the central regulatory protein of striated muscle contraction, and modulates the Ca2+-activation characteristics of muscle fibers. Troponin has 3 subunits, Troponin I(Tn-1), Troponin T(Tn-T) and Troponin C(Tn-C). Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. Mutations in all components of this complex have been associated with skeletal muscle disease. Recombinant human Troponin C was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: Troponin C protein, , Fast skeletal muscle troponin C protein, skeletal muscle protein, Troponin C protein, TNNC2 protein, Troponin C protein, Troponin C type 1 (slow) protein, skeletal muscle TNNC1 protein, Cardiac troponin C protein, Skeletal troponin C protein, slow skeletal and cardiac muscles protein, TNNC protein, Troponin C1 slow protein, Troponin C type 2 (fast) protein

Molecular Weight: 18.1 kDa (160 AA)