CD40 Protein (CD40)

Catalog No. ABIN935308

Product Details

Target: CD40

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Characteristics: Purified recombinant Human CD40 protein
Expression System: E.coli

Purity: > 98 % pure

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 5-10 mM Na3PO4, pH 7.5 to a concentration of 0.1-1.0 mg/mL.

Buffer: Sterile filtered through a 0.2 μm filter. Lyophilized from 10 mM Na3PO4, pH 7.5.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: CD40

Alternative Name: CD40 (CD40 Products)

Background: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L.
Alternative Names: soluble CD40 Ligand protein, CD-40 protein, CD-40, CD40, CD 40, TRAP protein, TNFSF5 protein, gp39 protein, T-BAM protein, CD154 protein, CD 40 protein

Molecular Weight: 16.3 kDa

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints