FAS Protein
Quick Overview for FAS Protein (ABIN935361)
Target
See all FAS ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS
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Characteristics
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Purified recombinant Human Fas Receptor protein
Expression System: E.coli
Bioactivity: The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/mL in the presence of 2ng/mL of hFasL. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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TNF Receptor Superfamily, Member 6 (FAS) (AA 25-169) protein (His-DYKDDDDK-Strep II Tag)
FAS Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
TNF Receptor Superfamily, Member 6 (FAS) protein (His tag)FAS Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
TNF Receptor Superfamily, Member 6 (FAS) protein (Fc Tag)FAS Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) protein (Fc Tag)FAS Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNF Receptor Superfamily, Member 6 (FAS) (AA 1-335) protein (GST tag)FAS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
TNF Receptor Superfamily, Member 6 (FAS) (AA 17-173) protein (His tag)FAS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) (Active) protein (His tag)FAS Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % SDS, AcA Active
TNF Receptor Superfamily, Member 6 (FAS) (Active) protein (His tag)FAS Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
TNF Receptor Superfamily, Member 6 (FAS) (Active) protein (His tag)FAS Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) (Active) protein (Fc Tag)FAS Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Supplied lyophilized with no additives.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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Alternative Name
- Fas Receptor
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Background
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Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.
Alternative Names: sFasR protein, soluble Fas receptor protein, Apo I protein, CD95 protein, Fas Antigen protein, TNFRSF6 protein -
Molecular Weight
- 17.6 kDa
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Pathways
- p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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