G-CSF Protein

Catalog No. ABIN935393

This Recombinant G-CSF protein is produced in Escherichia coli (E. coli).

Quick Overview for G-CSF Protein (ABIN935393)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

Characteristics: Purified recombinant Mouse GCSF protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-60 cells is 2 x 10^7 units/mg.

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL.

Buffer: Lyophilized from 5 mM sodium citrate, pH 4.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: GCSF

Background: G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and Mouse G-CSF are cross-species reactive.
Alternative Names: Granulocyte Colony-Stimulating Factor protein, MGI-1G protein, GM-CSFb protein, pluripoietin protein, CSF-3 protein

Molecular Weight: 19 kDa

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization