G-CSF Protein
Quick Overview for G-CSF Protein (ABIN935393)
Target
See all G-CSF (CSF3) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA
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Characteristics
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Purified recombinant Mouse GCSF protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-60 cells is 2 x 10^7 units/mg. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) protein
CSF3 Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) proteinCSF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-208) (Active) proteinCSF3 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) protein (His tag)CSF3 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 22-214) protein (His-IF2DI Tag)CSF3 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) protein (GST tag)CSF3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, AcA Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Lyophilized from 5 mM sodium citrate, pH 4.0.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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Alternative Name
- GCSF
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Background
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G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and Mouse G-CSF are cross-species reactive.
Alternative Names: Granulocyte Colony-Stimulating Factor protein, MGI-1G protein, GM-CSFb protein, pluripoietin protein, CSF-3 protein -
Molecular Weight
- 19 kDa
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
Target
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