GDF5 Protein

Catalog No. ABIN935396

This Recombinant GDF5 protein is produced in Escherichia coli (E. coli).

Quick Overview for GDF5 Protein (ABIN935396)

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

Characteristics: Purified recombinant Human GDF5 protein
Expression System: E.coli
Bioactivity: Induction of alkaline phosphatase activity in ATDC5 cells: EC50 = 40Nm.

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Assay Procedure:

Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of GDF5 as a Reference Standard.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Supplied as a lyophilized powder.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Alternative Name: GDF5

Background: GDF-5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type, brachydactyly, type C, and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.
Alternative Names: BMP-14 protein, LAP4 protein, Growth Differentiation Factor 5 protein, GDF 5 protein, GDF-5 protein, Cartilage-derived morphogenetic protein-1 protein, GDF-5 protein, Radotermin protein, CDMP1 protein, GDF5 protein, GDF 5, BMP14 protein, Growth differentiation factor 5 protein, GDF-5 protein, CDMP-1 protein, SYNS2 protein, CDMP1 protein, GDF5, GDF-5

Molecular Weight: 26.8 kDa