ALOX5 Protein
Quick Overview for ALOX5 Protein (ABIN935606)
Target
See all ALOX5 ProteinsProtein Type
Biological Activity
Origin
Source
-
-
Characteristics
-
Purified recombinant Human 5-Lipoxygenase protein
Expression System: Baculovirus
Bioactivity: 20 units/mg protein - One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25 °C in 50 mM Tris-HCl, 100 uM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 uM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System HEK-293 CellsConjugate His tagOrigin HumanPrice starts at $12,595.45Expression System Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin HumanPrice starts at $20,480.57Your project requires further customization? Contact us and discover our custom protein solutions
-
Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) protein (His tag)
custom-made ALOX5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) protein (His tag)custom-made ALOX5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) protein (GST tag)ALOX5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
-
-
Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
-
Restrictions
- For Research Use only
-
-
-
Buffer
- 50 mM Tris containing 0 mM EDTA, pH 8.0.
-
Handling Advice
- Avoid repeated freeze/thaw cycles.
-
Storage
- -80 °C
-
-
- ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))
-
Alternative Name
- 5-Lipoxygenase
-
Background
-
5-lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Alternative Names: 5-Lipoxygenase Antigen, 5 Lipoxygenase Recombinant Protein -
Molecular Weight
- 78 kDa
Target
-
