VEGF 165 (Active) Protein
Quick Overview for VEGF 165 (Active) Protein (ABIN935908)
Target
Protein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
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Characteristics
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Purified recombinant Human VEGF165 protein
Expression System: E.coli
Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/mL. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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VEGF 165 (AA 27-191) (Active) protein
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
VEGF 165 (AA 27-191) (Active) protein (Biotin,His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) (Active) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
VEGF 165 (AA 207-371) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
VEGF 165 (AA 207-371) proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
VEGF 165 (AA 27-191) protein (His tag)Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS
VEGF 165 proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Supplied as a lyophilized powder.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- VEGF 165
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Alternative Name
- VEGF165
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Background
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VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothellial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase(flt-1), kDaR gene product (the Mouse homolog of kDaR is the flk-1 gene product) and the flt4 gene product.
Alternative Names: Folliculostellate cell-derived growth factor protein, Glioma-derived endothelial cell mitogen protein, VEGF-165 protein, Vascular Endothelial Growth Factor protein, VPF protein -
Molecular Weight
- 38.5 kDa
Target
-
