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VEGF 165 (Active) Protein

Origin: Human Host: Escherichia coli (E. coli) Recombinant > 98 % pure Active

Catalog No. ABIN935908

Datasheet as PDF

Target

VEGF 165

Protein Type

Recombinant

Biological Activity

Active
Origin
19

2

1
Human
Source
9

7

2

1

1

1

1
Escherichia coli (E. coli)

Sequence

MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Characteristics

Purified recombinant Human VEGF165 protein
Expression System: E.coli
Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/mL.

Purity

> 98 % pure

Endotoxin Level

< 0.1 ng per µg (1 EU/µg).
VEGF 165 protein
Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)
Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active

VEGF 165 (AA 27-191) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (Homodimer) (Active) protein
Origin: Human Host: Insect Cells Recombinant > 95 % by SDS-PAGE Active

VEGF 165 (AA 207-371) protein (His tag)
Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS

VEGF 165 (AA 1-191) protein (His tag)
Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS

VEGF 165 protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS

VEGF 165 (Homodimer) (Active) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE Active

VEGF 165 (Active) protein
Origin: Mouse Host: Escherichia coli (E. coli) > 95 % by SDS-PAGE and HPLC analyses. Active

Application Notes

Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions

For Research Use only
Format

Lyophilized

Buffer

Supplied as a lyophilized powder.

Handling Advice

Avoid repeated freeze/thaw cycles.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Target

VEGF 165

Alternative Name

VEGF165

Background

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothellial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase(flt-1), kDaR gene product (the Mouse homolog of kDaR is the flk-1 gene product) and the flt4 gene product.
Alternative Names: Folliculostellate cell-derived growth factor protein, Glioma-derived endothelial cell mitogen protein, VEGF-165 protein, Vascular Endothelial Growth Factor protein, VPF protein

Molecular Weight

38.5 kDa