Avidin Protein (AVD) (TRITC)

Catalog No. ABIN964117

Supplier Product No.: a003-00

Native Avidin protein expressed in Chicken.

Quick Overview for Avidin Protein (AVD) (TRITC) (ABIN964117)

Target: Avidin (AVD)

Protein Type: Native

Origin: Chicken egg

Source: Chicken

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Product Details

Purification tag / Conjugate: This Avidin protein is labelled with TRITC.

Supplier Product No.: a003-00

Supplier: Rockland

Purpose: Avidin Rhodamine Conjugated

Purification: Avidin Rhodamine Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.

Application Details

Application Notes:

Flow Cytometry Dilution: 1:500 - 1:2,500

Application Note: Avidin Rhodamine Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.

FLISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Avidin (AVD)

Alternative Name: Avidin

Background:

Synonyms: Avidin Rhodamine Conjugated, Avidin TRITC Conjugated

Background: Avidin Rhodamine Conjugated is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P02701