Avidin Protein (AVD) (HRP)

Catalog No. ABIN964120

Supplier Product No.: a003-03

Native Avidin protein expressed in Chicken.

Quick Overview for Avidin Protein (AVD) (HRP) (ABIN964120)

Target: Avidin (AVD)

Protein Type: Native

Origin: Chicken egg

Source: Chicken

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Purification tag / Conjugate: This Avidin protein is labelled with HRP.

Supplier Product No.: a003-03

Supplier: Rockland

Purpose: Avidin Peroxidase Conjugated

Purification: Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single prec

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Avidin Peroxidase Conju Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Avidin (AVD)

Alternative Name: Avidin

Background:

Synonyms: HRP-Avidin conjugated, Horseradish peroxidase conjugated Avidin, AvD, HRP Avidin Biotin Complex, Avidin Peroxidase Conjugated, Avidin HRP Conjugated

Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the grea

UniProt: P02701