Avidin Protein (AVD) (Alkaline Phosphatase (AP))

Catalog No. ABIN964121

Supplier Product No.: a003-05

This Native Avidin protein is produced in Chicken.

Quick Overview for Avidin Protein (AVD) (Alkaline Phosphatase (AP)) (ABIN964121)

Target: Avidin (AVD)

Protein Type: Native

Origin: Chicken egg

Source: Chicken

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC)

Product Details

Purification tag / Conjugate: This Avidin protein is labelled with Alkaline Phosphatase (AP).

Supplier Product No.: a003-05

Supplier: Rockland

Purpose: Avidin Alkaline Phosphatase Conjugated

Purification: Avidin Alkaline Phosphatase Conjugated was prepared from electrophoretically pure Avidin from egg white followed by extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkal

Sterility: Sterile filtered

Application Details

Application Notes:

Flow Cytometry Dilution: 1:500 - 1:2,500

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Avidin Alkaline Phosphatase Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Avidin (AVD)

Alternative Name: Avidin

Background:

Synonyms: Avidin Alkaline Phosphatase Conjugated, Avidin alk phos Conjugated

Background: Avidin Alkaline Phosphatase Conjugated is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P02701