Avidin Protein (AVD) (Glucose Oxidase)

Catalog No. ABIN964122

Supplier Product No.: a003-07

This Native Avidin protein is expressed in Chicken.

Quick Overview for Avidin Protein (AVD) (Glucose Oxidase) (ABIN964122)

Target: Avidin (AVD)

Protein Type: Native

Origin: Chicken egg

Source: Chicken

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Purification tag / Conjugate: This Avidin protein is labelled with Glucose Oxidase.

Supplier Product No.: a003-07

Supplier: Rockland

Purpose: Avidin Glucose Oxidase Conjugated

Purification: Avidin Glucose Oxidase was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and an

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated has been tested by ELISA and can be utilized in both Western Blotting and ELISA experiment formats in combinati

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Avidin (AVD)

Alternative Name: Avidin

Background:

Synonyms: AvD, Avidin Biotin Complex, GOD, GOx, Avidin Glucose Oxidase Conjugated

Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the grea

UniProt: P02701