Biotin Protein (HRP)

Catalog No. ABIN964124

Supplier Product No.: b000-03

This Biotin protein is produced in Synthetic.

Quick Overview for Biotin Protein (HRP) (ABIN964124)

Target: Biotin

Origin: Chemical

Source: Synthetic

Application: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Purification tag / Conjugate: This Biotin protein is labelled with HRP.

Supplier Product No.: b000-03

Supplier: Rockland

Purpose: Biotin Peroxidase Conjugated

Purification: Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Biotin Peroxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 5.0 mL)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Peroxidase Conju Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Biotin

Target Type: Chemical

Background:

Synonyms: Vitamin H, Coenzyme R, Vitamin B7, Biotin Peroxidase Conjugated, Biotin HRP Conjugated

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav