Biotin Protein (HRP)
Quick Overview for Biotin Protein (HRP) (ABIN964124)
Target
Origin
Source
Application
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Purification tag / Conjugate
- This Biotin protein is labelled with HRP.
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Supplier Product No.
- b000-03
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Supplier
- Rockland
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Specificity
- Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.
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Application Notes
- Biotin Peroxidase Conjugated can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
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Comment
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Biotin Peroxidase Conjugated can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Preservative
- Gentamicin sulfate
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Handling Advice
- Do NOT add Sodium Azide!
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Storage
- 4 °C
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Expiry Date
- 12 months
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- Biotin
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Target Type
- Chemical
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Background
- Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin
Target
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