Biotin Protein (Alkaline Phosphatase (AP))

Catalog No. ABIN964125

Supplier Product No.: b000-05

This Biotin protein is expressed in Synthetic.

Quick Overview for Biotin Protein (Alkaline Phosphatase (AP)) (ABIN964125)

Target: Biotin

Origin: Chemical

Source: Synthetic

Application: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Purification tag / Conjugate: This Biotin protein is labelled with Alkaline Phosphatase (AP).

Supplier Product No.: b000-05

Supplier: Rockland

Purpose: Biotin Alkaline Phosphatase Conjugated

Purification: This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Biotin Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Biotin

Target Type: Chemical

Background:

Synonyms: Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav