Biotin Protein (Glucose Oxidase)

Catalog No. ABIN964126

Supplier Product No.: b000-07

Biotin protein expressed in Synthetic.

Quick Overview for Biotin Protein (Glucose Oxidase) (ABIN964126)

Target: Biotin

Origin: Chemical

Source: Synthetic

Application: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Microarray (MA)

Product Details

Purification tag / Conjugate: This Biotin protein is labelled with Glucose Oxidase.

Supplier Product No.: b000-07

Supplier: Rockland

Purpose: Biotin Glucose Oxidase Conjugated

Purification: Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin.  Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Biotin Glucose Oxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 5.0 mL)

Concentration: 1.0 mg/mL

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Glucose Oxidase Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Biotin

Target Type: Chemical

Background:

Synonyms: Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav