Biotin Protein (beta-Gal)

Catalog No. ABIN964127

Supplier Product No.: b000-17

This Biotin protein is produced in Synthetic.

Quick Overview for Biotin Protein (beta-Gal) (ABIN964127)

Target: Biotin

Origin: Chemical

Source: Synthetic

Application: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Purification tag / Conjugate: This Biotin protein is labelled with beta-Gal.

Supplier Product No.: b000-17

Supplier: Rockland

Purpose: Biotin Beta Galactosidase Conjugated

Purification: Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin.   Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and an

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Biotin Beta Galactosidase Conjugated has been tested by ELISA and dot blot and is assayed against 1.0 μg of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A wor

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol, pH 7.8

Handling Advice: DO NOT FREEZE. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening.   DO NOT FREEZE.   This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Biotin

Target Type: Chemical

Background:

Synonyms: Vitamin H, Coenzyme R, Vitamin B7, β-galactosidase, β-gal, beta-gal, Biotin Beta Galactosidase Conjugated, Biotin Beta Gal Conjugated

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav