Biotin Protein (beta-Gal)

Catalog No. ABIN964127

Supplier Product No.: b000-17

This Biotin protein is produced in Synthetic.

Quick Overview for Biotin Protein (beta-Gal) (ABIN964127)

Target: Biotin

Origin: Chemical

Source: Synthetic

Application: ELISA

Product Details

Purification tag / Conjugate: This Biotin protein is labelled with beta-Gal.

Supplier Product No.: b000-17

Supplier: Rockland

Specificity: Biotin Beta Galactosidase Conjugated has been assayed against 1.0 μg of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 to 1:3, 000 is suggested for Biotin Beta Galactosidase Conjugated. Biotin Beta Galactosidase Conjugated has been assayed against 1.0 μg of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 is suggested for Biotin Beta Galactosidase Conjugated.

Application Details

Application Notes: Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.  Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.

Comment:

Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 to 1:3, 000 is suggested for Biotin Beta Galactosidase Conjugated. Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 is suggested for Biotin Beta Galactosidase Conjugated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.05 M Potassium Phosphate, 0.001 M Magnesium Chloride, 0.1 M Beta-Mercaptoethanol, pH 7.8

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Biotin

Target Type: Chemical

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin