Streptavidin Protein
Quick Overview for Streptavidin Protein (ABIN964535)
Target
See all Streptavidin ProteinsProtein Type
Origin
Host
Application
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Supplier Product No.
- s000-01
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Supplier
- Rockland
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Specificity
- Streptavidin is chromatographically pure and shows predominantly a single 53,600 dalton band by SDS-PAGE.
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Characteristics
- Specific Activity Value: 17.6 U/mg by biotin titration method
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Application Notes
- Streptavidin is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes Southern blots and other methodologies related to DNA and RNA analysis Western blots and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.
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Comment
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Streptavidin is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes, Southern blots and other methodologies related to DNA and RNA analysis, Western blots, and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
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Concentration
- 0.9 mg/mg
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Buffer
- 0.15 M Sodium Chloride
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Storage
- 4 °C
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Expiry Date
- 12 months
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- Streptavidin
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Background
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Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments.
Synonyms: SA, S avidin, streptococcus avidin -
UniProt
- P22629
Target
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