Streptavidin Protein (HRP)

Catalog No. ABIN964537

Supplier Product No.: s000-03

Native Streptavidin protein expressed in Streptomyces avidinii and has been mentioned in 4+ publications.

Quick Overview for Streptavidin Protein (HRP) (ABIN964537)

Target: Streptavidin

Protein Type: Native

Origin: Streptomyces avidinii

Source: Streptomyces avidinii

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Fluorescence Microscopy (FM)

Product Details

Purification tag / Conjugate: This Streptavidin protein is labelled with HRP.

Supplier Product No.: s000-03

Supplier: Rockland

Purpose: Streptavidin Peroxidase Conjugated

Purification: Streptavidin-HRP was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Bl

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Peroxidase Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

References

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

Vasek, Garber, Dorsey, Durrant, Bollman, Soung, Yu, Perez-Torres, Frouin, Wilton, Funk, DeMasters, Jiang, Bowen, Mennerick, Robinson, Garbow, Tyler, Suthar, Schmidt, Stevens, Klein: "A complement-microglial axis drives synapse loss during virus-induced memory impairment." in: Nature, Vol. 534, Issue 7608, pp. 538-43, (2016) (PubMed).

Cantoni, Bollman, Licastro, Xie, Mikesell, Schmidt, Yuede, Galimberti, Olivecrona, Klein, Cross, Otero, Piccio: "TREM2 regulates microglial cell activation in response to demyelination in vivo." in: Acta neuropathologica, Vol. 129, Issue 3, pp. 429-47, (2015) (PubMed).

Cowles, Li, Semmelhack, Cristea, Silhavy: "The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli." in: Molecular microbiology, Vol. 79, Issue 5, pp. 1168-81, (2011) (PubMed).

Target Details

Target: Streptavidin

Background:

Synonyms: HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin

Background: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, im

UniProt: P22629