Streptavidin Protein (Alkaline Phosphatase (AP))

Catalog No. ABIN964538

Supplier Product No.: s000-05

This Native Streptavidin protein is produced in Streptomyces avidinii.

Quick Overview for Streptavidin Protein (Alkaline Phosphatase (AP)) (ABIN964538)

Target: Streptavidin

Protein Type: Native

Origin: Streptomyces avidinii

Source: Streptomyces avidinii

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details

Purification tag / Conjugate: This Streptavidin protein is labelled with Alkaline Phosphatase (AP).

Supplier Product No.: s000-05

Supplier: Rockland

Purpose: Streptavidin Alkaline Phosphatase Conjugated

Purification: Streptavidin-Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin.   Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf inte

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Streptavidin Alkaline Phosphatase conjugated has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment

Western Blot Dilution: 1:1,000 - 1:4,000

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE. Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss o Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Streptavidin

Background:

Synonyms: SA alkaline phosphatase conjugate, S avidin conjugated to alkaline phosphatase, alkaline phosphatase conjugated to streptococcus avidin, streptavidin Alk Phos, SA-ALP, ALP conjugated streptavidin

Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditio

UniProt: P22629