Streptavidin Protein (PE)

Catalog No. ABIN964539

Supplier Product No.: s000-08

This Native Streptavidin protein is expressed in Streptomyces avidinii.

Quick Overview for Streptavidin Protein (PE) (ABIN964539)

Target: Streptavidin

Protein Type: Native

Origin: Streptomyces avidinii

Source: Streptomyces avidinii

Application: ELISA, Fluorescence Microscopy (FM), Flow Cytometry (FACS), Multiplex Assay (MA), Dot Blot (DB), Immunofluorescence (IF), Biochemical Assay (BCA), Functional Studies (Func)

Product Details

Purification tag / Conjugate: This Streptavidin protein is labelled with PE.

Supplier Product No.: s000-08

Supplier: Rockland

Purpose: Streptavidin Phycoerythrin Conjugated

Purification: Streptavidin-Phycoerythrin was prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin.   Free fluorochrome is removed by tandem molecular sieve chromatography.

Sterility: Sterile filtered

Application Details

Application Notes:

Flow Cytometry Dilution: 1:100 - 1:250

Application Note: Phycoerythrin conjugated streptavidin has been tested by dot blot and FACS and is suitable for immunomicroscopy, flow cytometry or FACS analysis, as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c)

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room tempe Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Streptavidin

Background:

Synonyms: Streptavidin PE, Phyco Streptavidin, SA Phycoerythrin, R-Phycoerythrin, Phycoerythrin conjugated streptavidin

Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditio

UniProt: P22629