Streptavidin Protein (beta-Gal)
Quick Overview for Streptavidin Protein (beta-Gal) (ABIN964542)
Target
See all Streptavidin ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This Streptavidin protein is labelled with beta-Gal.
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Supplier Product No.
- s000-17
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Supplier
- Rockland
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Purpose
- Streptavidin Beta-Galactosidase Conjugated
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Purification
- This product was prepared from electrophoretically pure Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-beta galactosidase (E.coli) and anti-Streptavidin. No reaction was observed against anti-Avidin.
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Sterility
- Sterile filtered
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Streptavidin protein (HRP)
Origin: Streptomyces avidinii Host: Streptomyces avidinii Native WB, ELISA, FM, IHC, DB, IF, IEM
Streptavidin protein (PE)Origin: Streptomyces avidinii Host: Streptomyces avidinii Native ELISA, FM, FACS, MA, DB, IF, BCA, Func
Streptavidin (AA 25-183) protein (His tag)Origin: Streptomyces Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Streptavidin (AA 37-159) protein (His tag)Origin: Streptomyces Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Streptavidin protein (TRITC)Origin: Streptomyces avidinii Host: Streptomyces avidinii Native FM, FLISA, IHC, FISH
Streptavidin protein (Atto 425)Origin: Streptomyces avidinii Host: Streptomyces avidinii Native WB, FM, FLISA, DB, IF
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Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Streptavidin Beta Galactosidase conjugate has been tested by ELISA and is assayed against 1.0 μg of biotinylated IgG in a standard capture ELISA using ONPG as a substrate for 30 minutes at room temperature. A working dilution of 1:100 to 1:3,000 is sugge
Western Blot Dilution: 1:400 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01M Magnesium Chloride and 0.01M Beta-Mercaptoethanol
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- DO NOT FREEZE.
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Storage
- 4 °C
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Storage Comment
- Store vial at 4° C prior to opening. DO NOT FREEZE. Expiration date is one (1) year from date of receipt.
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Expiry Date
- 12 months
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- Streptavidin
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Background
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Synonyms: SA, S avidin, streptococcus avidin, streptavidin Beta Galactosidase [E.coli], BGAL
Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin. β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosa
Target
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