Transferrin Protein (TF)

Catalog No. ABIN964544

Supplier Product No.: 009-0134

This Native Transferrin protein is produced in Human.

Quick Overview for Transferrin Protein (TF) (ABIN964544)

Target: Transferrin (TF)

Protein Type: Native

Origin: Human

Source: Human

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Product Details

Supplier Product No.: 009-0134

Supplier: Rockland

Purpose: Human Transferrin- 009-0134

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Transferrin and anti-Human Serum.

Characteristics: apotransferrin, Transferrin Control, Siderophilin, TRF, apo-hTF

Purification: Human transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis. 

Application Details

Application Notes:

Application Note: Human Apo Transferrin from human serum was lyophilized from 0.2M ammonium bicarbonate for use in cell culture and assays. Transferrin has been tested in SDS-Page and is also suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified proteins.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:10,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 1.0 mL

Concentration: 10.0 mg/mL

Buffer:

Buffer: See application note.

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin-

Background: Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality.

Gene ID: 7018

UniProt: Q06AH7

Pathways: Transition Metal Ion Homeostasis