Transferrin Protein (TF) (AA 20-698) (His tag)

Catalog No. ABIN7275694

Product Details

Target: Transferrin (TF)

Protein Type: Recombinant

Protein Characteristics: AA 20-698

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Transferrin protein is labelled with His tag.

Purpose: Human Transferrin Protein

Sequence: Val20-Pro698

Characteristics: Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Pro698.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin (TF Products)

Synonyms: ltf Protein, pro1557 Protein, pro2086 Protein, mgc107777 Protein, LOC692564 Protein, TF Protein, LOC100144362 Protein, tf Protein, LTF Protein, TFEW Protein, conalbumin Protein, tf-b Protein, MGC64306 Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, AI266983 Protein, Cd176 Protein, HP Protein, Tf Protein, Tfn Protein, hpx Protein, Trf Protein, cb285 Protein, gavi Protein, id:ibd3238 Protein, id:ibd3525 Protein, sb:cb285 Protein, wu:fb57g06 Protein, wu:fb62h02 Protein, wu:fb63h10 Protein, wu:fb64h10 Protein, zgc:112154 Protein, 143958_at Protein, CG6186 Protein, Dmel\\CG6186 Protein, TSF1 Protein, anon-EST:Posey265 Protein, tsf1 Protein, Pro-TRH Protein, IL-5 Protein, STF I Protein, TRF1 Protein, sTF1 Protein, sTf Protein, tf1 Protein, transferrin Protein, serotransferrin Protein, transferrin (ovotransferrin) Protein, transferrin L homeolog Protein, melanotransferrin Protein, transferrin-a Protein, Transferrin 1 Protein, thyrotropin releasing hormone Protein, interleukin 5 Protein, TF Protein, LOC477072 Protein, tf Protein, Tf Protein, LOC100144362 Protein, tf.L Protein, LOC5575625 Protein, Trf Protein, tfa Protein, Tsf1 Protein, TRH Protein, IL5 Protein, LOC101085148 Protein, LOC100726872 Protein, trf Protein

Background: Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain, i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.

Molecular Weight: 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Tris-Bis PAGE result.

Pathways: Transition Metal Ion Homeostasis