Transferrin Protein (TF) (FITC)

Catalog No. ABIN964545

Supplier Product No.: 009-0234

This Native Transferrin protein is expressed in Human.

Quick Overview for Transferrin Protein (TF) (FITC) (ABIN964545)

Target: Transferrin (TF)

Protein Type: Native

Origin: Human

Source: Human

Application: Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details

Purification tag / Conjugate: This Transferrin protein is labelled with FITC.

Supplier Product No.: 009-0234

Supplier: Rockland

Purpose: Human Transferrin Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human Transferrin and anti-Human Serum.

Characteristics: Human transferrin fluorescein conjugation, FITC conjugated transferrin

Purification: This product was prepared from normal serum by delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  

Application Details

Application Notes:

Application Note: Human transferrin fluorescein conjugation has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

IF Microscopy Dilution: 1:500-1:2500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin

Background: Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality.

Gene ID: 7018

UniProt: Q06AH7

Pathways: Transition Metal Ion Homeostasis