Transferrin Protein (TF) (Biotin)

Catalog No. ABIN964547

Supplier Product No.: 009-0634

This Native Transferrin protein is produced in Human.

Quick Overview for Transferrin Protein (TF) (Biotin) (ABIN964547)

Target: Transferrin (TF)

Protein Type: Native

Origin: Human

Source: Human

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunohistochemistry (IHC), Immunomicroscopy (IM)

Product Details

Purification tag / Conjugate: This Transferrin protein is labelled with Biotin.

Supplier Product No.: 009-0634

Supplier: Rockland

Purpose: Human Transferrin Biotin Conjugation

Purification: This product was prepared from normal serum by a multi-stage process that includes delipidation and selective precipitation.

Protein Source: normal human serum

Application Details

Application Notes: Human Transferrin Biotin has been tested in dot blot and is designed for Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.

Comment:

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Transferrin and anti-Human Serum.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with 1.0 mL deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000), 0.01% (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin

Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Gene ID: 7018

UniProt: Q06AH7

Pathways: Transition Metal Ion Homeostasis