NRG-1 EGF-Like Domain (NRG-1beta2) (Active) Protein

Catalog No. ABIN988181

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for NRG-1 EGF-Like Domain (NRG-1beta2) (Active) Protein (ABIN988181)

Target: NRG-1 EGF-Like Domain (NRG-1beta2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Product Details

Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using serum free human MCF-7 cells is less than 83 ng/ml, corresponding to a specific activity of >, 1.2 × 104 IU/mg.

Endotoxin Level: Level Less than 1EU/µg of rHuNRG-1 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: NRG-1 EGF-Like Domain (NRG-1beta2)

Background: Neuregulin is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells, playing an important role in heart structure and function through inducing ErbB2/ErbB4 receptor phosphorylation and cardiomyocyte differentiation. Research on molecular level discovered that recombinant neuregulin could make disturbed myocardial cell structure into order and strengthen the connection between myocardial cells by intercalated discs re-organization. Synonym: NRG-1 EGF-like domain (NRG-1beta2), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight: 7,005 Da, a single non-glycosylated polypeptide chain containing 61 amino acids.