CCL17 Protein

Catalog No. ABIN988251

This Recombinant CCL17 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL17 Protein (ABIN988251)

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Product Details

Sequence: ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

Endotoxin Level: Level Less than 1EU/µg of rHuTARC/CCL17 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: TARC/CCL17

Background: CCL17 is a novel CC chemokine recently identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1ª, MIP-1beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was recently identified to be a specific functional ligand for CCR-4, a receptor that is selectively expressed on T cells. Synonym: TARC/CCL17, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Molecular Weight: 8.0kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.