RBOHD antibody
The Rabbit Polyclonal anti-RBOHD antibody (ABIN4966293) specifically detects RBOHD in IF and WB.
The antibody is reactive with Arabidopsis thaliana and Tomato samples.
Catalog No. ABIN4966293
$646.39
Plus shipping costs $50.00
50 μg
Shipping to:
United States
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Quick Overview for RBOHD antibody (ABIN4966293)
Target
RBOHD
(Respiratory Burst Oxidase-D (RBOHD))
Reactivity
Arabidopsis thaliana, Tomato
Host
Rabbit
Clonality
Polyclonal
Application
Immunofluorescence (IF), Western Blotting (WB)
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Purpose
- Anti-RBOHD | Respiratory burst oxidase homolog protein D
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Cross-Reactivity (Details)
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Arabidopsis thaliana, Solanum sp.
Not reactive in: Marchantia polymorpha, Medicago sativa -
Predicted Reactivity
- Brassica napus,Citrus sinensis,Citrullus colocynthis,Cucumis melo,Fragaria ananassa,Gossypium arboreum,Hordeum vulgare,Manihot esculenta,Morus notabilis,Nicotiana tabacum,Oryza sativa,Picea abies,Ricinus communis,Sinensis osbeck,Solanum tuberosum,Theobroma cacao,Triticum aestivum,Zea mays,Zostera marina
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Purification
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated peptide chosen from Arabidopsis thaliana RBOHD sequence
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Application Notes
- 1 : 1000 (WB)
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Comment
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RBOHD is a protein with 6 transmembrane-spanning regions, heat denaturation will cause protein aggregation. Therefore 30 minutes at RT was applied in the experiment below.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- RBOHD (Respiratory Burst Oxidase-D (RBOHD))
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Alternative Name
- Respiratory burst oxidase homolog protein D (RBOHD)
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Background
- RBOHD (Respiratory burst oxidase homolog protein D) is a Calcium-dependent NADPH oxidase that generates superoxide. Is involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling.
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Molecular Weight
- 104 kDa
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Gene ID
- 834842
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UniProt
- Q9FIJ0
Target
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