anti-Bat Metabotropic Glutamate Receptor 5 antibody for Immunohistochemistry

Recommended Metabotropic Glutamate Receptor 5 Antibody (supplied by: Log in to see )

Antigen
Glutamate Receptor, Metabotropic 5 (GRM5) Antibodies
  • GPRC1E
  • MGLUR5
  • mGlu5
  • mGluR5
  • mGlur5
  • 6430542K11Rik
  • AI850523
  • Glu5R
  • Gprc1e
  • mGluR5b
  • glutamate receptor, metabotropic 5
  • glutamate metabotropic receptor 5
  • GRM5
  • Grm5
Epitope
C-Term
70
60
15
14
12
7
4
3
3
3
2
2
2
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus)
185
131
120
6
6
5
5
2
2
2
2
2
1
Host
Rabbit
97
89
4
2
Clonality
Polyclonal
Conjugate
This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
7
7
7
6
6
6
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
103
71
57
48
38
37
21
13
12
4
3
2
1
1
Options
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Available images

Catalog No. ABIN1048937
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.973619 ABIN213594 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
6.97362 ABIN2469508 IHC Rabbit N-Term Log in to see Polyclonal 0
-8.159155 ABIN4334124 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Glutamate Receptor, Metabotropic 5 (GRM5) Antibodies
Epitope C-Term
(70), (60), (15), (14), (12), (7), (4), (3), (3), (3), (2), (2), (2), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus)
(185), (131), (120), (6), (6), (5), (5), (2), (2), (2), (2), (2), (1)
Host Rabbit
(97), (89), (4), (2)
Clonality
Polyclonal
Conjugate This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(158), (103), (71), (57), (48), (38), (37), (21), (13), (12), (4), (3), (2), (1), (1)
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Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Target Details Metabotropic Glutamate Receptor 5 Application Details Handling Images
Brand IHC-plus™
Specificity Human GRM5 / MGLUR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PLEC (45 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%) Platypus (95%) Opossum, Turkey, Chicken, Lizard (90%).
Purification Immunoaffinity purified
Immunogen Synthetic 20 amino acid peptide from C-terminus of human GRM5 / MGLUR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%), Platypus (95%), Opossum, Turkey, Chicken, Lizard (90%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details Metabotropic Glutamate Receptor 5

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRM5 / MGLUR5 (GRM5 Antibody Abstract)
Background Name/Gene ID: GRM5
Subfamily: Metabotropic glutamate
Family: GPCR

Synonyms: GRM5, GPRC1E, MGLUR5, Mglur5b, MGlu5
Gene ID 2915
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Handling Images back to top
Application Notes Approved: IHC, IHC-P (4 - 13 μg/mL), WB
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Glutamate Receptor, Metabotropic 5 (GRM5) (C-Term) antibody (ABIN1048937) Anti-GRM5 / MGLUR5 antibody ABIN1048937 IHC staining of human brain, neurons and gli...
Immunohistochemistry (IHC) image for anti-Glutamate Receptor, Metabotropic 5 (GRM5) (C-Term) antibody (ABIN1048937) Anti-GRM5 / MGLUR5 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of...