RGL2 antibody
This Rabbit Polyclonal antibody specifically detects RGL2 in WB. It exhibits reactivity toward Arabidopsis thaliana.
Catalog No. ABIN4966294
Quick Overview for RGL2 antibody (ABIN4966294)
Target
RGL2
(DELLA Protein RGL2 (RGL2))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: Triticum sp.
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Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450
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Application Notes
- 1 : 1000 with standard ECL (WB) on transfected Arabidopsis thaliana protoplasts
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Comment
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As this protein is expressed in tissue in very low levels, please load at least 50 μg/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 μl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- RGL2 (DELLA Protein RGL2 (RGL2))
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Alternative Name
- RGA-like protein 2 (DELLA protein) (RGL2)
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Background
- DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
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Molecular Weight
- 60 kDa
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UniProt
- Q8GXW1
Target
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