Goat anti-Human IgA Antibody

Catalog No. ABIN100715

Supplier Product No.: 609-1106

Quick Overview for Goat anti-Human IgA Antibody (ABIN100715)

Target: IgA

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Supplier Product No.: 609-1106

Supplier: Rockland

Purpose: Human IgA (alpha chain) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against Human IgM or Human IgG.   ELISA was used to confirm minimal cross reactivity against other human heavy or light chain isotypes.

Characteristics: Anti-Human IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 fragment antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.

Purification: Goat Anti-Human IgA was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Human IgA alpha heavy chain

Application Details

Application Notes: Application Note: Human secondary antibody to IgA is available in a variety of formats. Anti-Human IgA Antibody has been tested by ELISA and dot blot and is suitable for Immunohistochemistry, Western Blotting as well as other antibody based assays. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:80,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.23 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store secondary antibody at 4° C prior to opening.  This Anti-Human IgA is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: IgA

Target Type: Antibody

Background: Anti-Human IgA Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.