Goat anti-Human IgA, IgG, IgM Antibody

Catalog No. ABIN100788

Supplier Product No.: 609-101-130

Product Details

Target: IgA, IgG, IgM

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 609-101-130

Supplier: Rockland

Purpose: Human IgG IgA IgM (H&L) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.

Characteristics: Anti-Human IgG (H&L) generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Human IgG, IgA and IgM whole molecules

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Human IgG IgA IgM antibody has been tested by ELISA and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:28,800 - 1:48,800 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG,IgA,IgM (IgA, IgG, IgM Products)

Target Type: Antibody

Background: Anti-Human IgG IgA IgM (H&L) Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.