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Goat anti-Mouse IgA, IgG, IgM Antibody (TRITC) - Preadsorbed

Rockland 610-100-430 FACS, FM, FLISA Polyclonal IgG TRITC
Rockland
Catalog No. ABIN100816
Supplier Product No.: 610-100-430
  • Target See all IgA, IgG, IgM products
    IgA, IgG, IgM
    Reactivity
    • 72
    • 48
    • 33
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Mouse
    Host
    • 159
    • 55
    • 4
    • 3
    • 2
    Goat
    Clonality
    • 215
    • 2
    Polyclonal
    Conjugate
    • 54
    • 38
    • 32
    • 27
    • 21
    • 10
    • 10
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    TRITC
    Application
    • 117
    • 113
    • 79
    • 77
    • 68
    • 56
    • 26
    • 22
    • 22
    • 21
    • 19
    • 17
    • 16
    • 15
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
    Supplier Product No.
    610-100-430
    Supplier
    Rockland
    Purpose
    Mouse IgG IgA IgM (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed
    Cross-Reactivity (Details)
    Minimal crossreactivity against Bv, Hs, Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  No reaction was observed against bovine, horse or human serum proteins.  This reagent is suitable for the detection of all mouse immunoglobulin isotypes and chain combinations.
    Characteristics
    Anti-MOUSE IgG F(ab')2 Antibody Rhodamine Conjugated generated in goat recognizes the dimeric Fab portion of the mouse IgG molecule. MOUSE IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG F(ab')2 Antibody Rhodamine Conjugated is ideal for investigators involved in serum protein components.
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen
    Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
    Isotype
    IgG
  • Application Notes
    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgA, IgG, IgM
    Alternative Name
    IgG,IgA,IgM (IgA, IgG, IgM Products)
    Target Type
    Antibody
    Background
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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