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Rabbit anti-Cow IgG (Fc Region) Antibody

ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
Catalog No. ABIN100938
Supplier Product No.: 601-4103

Quick Overview for Rabbit anti-Cow IgG (Fc Region) Antibody (ABIN100938)

Target

IgG

Clonality

  • 3437
  • 230
  • 16
  • 1
Polyclonal

Application

  • 2118
  • 2049
  • 1885
  • 1872
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype

IgG
  • Binding Specificity

    • 2397
    • 516
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Fc Region

    Reactivity

    • 753
    • 703
    • 607
    • 468
    • 410
    • 244
    • 213
    • 175
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow

    Host

    • 1773
    • 1582
    • 567
    • 282
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Rabbit

    Supplier Product No.

    601-4103

    Supplier

    Rockland

    Purpose

    Bovine IgG Fc Antibody

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum. No reaction was observed against Bovine IgG F(ab).

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Sterility

    Sterile filtered

    Immunogen

    Immunogen: Anti-Bovine IgG F(c) was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.

    Immunogen Type: Native Protein

  • Application Notes

    Application Note: Anti-Bovine IgG F(c) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    2.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target

    IgG

    Target Type

    Antibody

    Background

    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
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