Rabbit anti-Guinea Pig IgG (Fc Region) Antibody
Quick Overview for Rabbit anti-Guinea Pig IgG (Fc Region) Antibody (ABIN101333)
Target
Clonality
Application
Isotype
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Binding Specificity
- Fc Region
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Reactivity
- Guinea Pig
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Host
- Rabbit
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Supplier Product No.
- 606-4103
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Supplier
- Rockland
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Purpose
- Guinea Pig IgG Fc Antibody
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab) .
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Characteristics
- Anti-Guinea Pig IgG Antibody generated in rabbit detects guinea pig IgG.
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Sterility
- Sterile filtered
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Immunogen
- Optional[Immunogen]: Guinea Pig IgG F(c) fragment
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Application Notes
- Application Note: Anti-Guinea Pig IgG F(c) antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.69 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Receptors bind the Fc portion of guinea pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Target
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