Goat anti-Monkey IgG Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN101677

Supplier Product No.: 617-105-012

Quick Overview for Goat anti-Monkey IgG Antibody (Alkaline Phosphatase (AP)) (ABIN101677)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Monkey

Host: Goat

Supplier Product No.: 617-105-012

Supplier: Rockland

Purpose: Monkey IgG (gamma chain) Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Monkey IgG and Monkey Serum. 

Characteristics: Anti-Monkey IgM (mu chain) alkaline phosphatase antibody generated in goat specifically detects monkey IgM.

Purification: Monkey IgG gamma chain specific antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Monkey IgG gamma heavy chain

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Monkey IgG alkaline phosphatase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

References

Pollara, Jones, Huffman, Edwards, Dennis, Li, Jha, Goodman, Kumar, LaBranche, Montefiori, Fouda, Hope, Tomaras, Staats, Ferrari, Permar: "Bridging Vaccine-Induced HIV-1 Neutralizing and Effector Antibody Responses in Rabbit and Rhesus Macaque Animal Models." in: Journal of virology, Vol. 93, Issue 10, (2019) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Monkey IgG alkaline phosphatase conjugated antibody generated in goat detects specifically Monkey IgG. This secondary alkaline phosphatase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.