Donkey anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN101709

Supplier Product No.: 610-703-124

Quick Overview for Donkey anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN101709)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Donkey

Supplier Product No.: 610-703-124

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Characteristics: Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L).

Purification: Mouse IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other peroxidase antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:20,000 - 1:80,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Czulkies, Mastroianni, Lutz, Lang, Schwan, Schmidt, Lassmann, Zeiser, Aktories, Papatheodorou: "Loss of LSR affects epithelial barrier integrity and tumor xenograft growth of CaCo-2 cells." in: Oncotarget, Vol. 8, Issue 23, pp. 37009-37022, (2018) (PubMed).

Schorch, Heni, Zahaf, Brummer, Mione, Schmidt, Papatheodorou, Aktories: "Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL." in: Oncotarget, Vol. 9, Issue 23, pp. 16489-16500, (2018) (PubMed).

Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).

Hemmasi, Czulkies, Schorch, Veit, Aktories, Papatheodorou: "Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR)." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14031-44, (2015) (PubMed).

Litjens, Nguyen, Castro, Aromataris, Jones, Barritt, Rychkov: "Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells." in: The Biochemical journal, Vol. 405, Issue 2, pp. 269-76, (2007) (PubMed).

Dazy, Damiola, Parisey, Beug, Gandrillon: "The MEK-1/ERKs signalling pathway is differentially involved in the self-renewal of early and late avian erythroid progenitor cells." in: Oncogene, Vol. 22, Issue 58, pp. 9205-16, (2004) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.