Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Biotin)

Catalog No. ABIN101740

Supplier Product No.: 610-1602

Quick Overview for Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Biotin) (ABIN101740)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-1602

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum.

Characteristics: Anti-Mouse IgG ATTO 655 Antibody generated in goat detects reactivity to Mouse IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

Immunogen:

Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse IgG (H&L) biotin conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:350,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Mouse IgG (H&L) biotin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.