Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN101776

Supplier Product No.: 610-4102

Quick Overview for Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (ABIN101776)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Supplier Product No.: 610-4102

Supplier: Rockland

Purpose: Mouse IgG (H&L) Secondary Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Characteristics: Anti-Mouse IgG Texas Red Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3.

Purification: Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Application Details

Application Notes: Application Note: Anti-Mouse IgG Antibody has been tested by ELISA and is ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:400,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.08 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References

Stamatiades, Tremblay, Bohm, Crozet, Bisht, Kao, Coelho, Fan, Yewdell, Davidson, Heeger, Diebold, Nimmerjahn, Geissmann: "Immune Monitoring of Trans-endothelial Transport by Kidney-Resident Macrophages." in: Cell, Vol. 166, Issue 4, pp. 991-1003, (2016) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.