Goat anti-Mouse IgG (Fc Region) Antibody

Catalog No. ABIN101851

Supplier Product No.: 110-1103

Quick Overview for Goat anti-Mouse IgG (Fc Region) Antibody (ABIN101851)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Supplier Product No.: 110-1103

Supplier: Rockland

Purpose: Mouse IgG Fc Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against Mouse IgG F(ab).

Characteristics: MOUSE IgG (H&L) (GOAT) Antibody generated in sheep detects specifically mouse IgG whole molecule. Anti-mouse IgG antibody is ideal for investigators involved in serum protein component research.

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Optional[Immunogen]: Mouse IgG F(c) fragment

Application Details

Application Notes: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Li, Wang, Li: "Kinesin family member 20B regulates tongue cancer progression by promoting cell proliferation." in: Molecular medicine reports, Vol. 19, Issue 3, pp. 2202-2210, (2019) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.