Goat anti-Pig IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN101876

Supplier Product No.: 614-1502

Quick Overview for Goat anti-Pig IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN101876)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Pig

Host: Goat

Supplier Product No.: 614-1502

Supplier: Rockland

Purpose: Swine IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Swine IgG and Swine Serum.

Characteristics: Anti-Swine IgG whole molecule antibody generated in goat detects specifically Swine IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Swine IgG Alkaline Phosphatase Conjugated Antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Swine IgG whole molecule antibody generated in goat detects specifically Swine IgG whole molecule. This secondary antibody anti-Swine is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.